Characterization of extended-spectrum and CMY-2 ß -lactamases, and associated virulence genes in Escherichia coli from food of animal origin in México
ISSN: 0007-070X
Article publication date: 7 August 2018
Issue publication date: 7 August 2018
Abstract
Purpose
Broad-spectrum cephalosporin resistance is rapidly increasing in Escherichia coli, representing a food safety problem. The purpose of this paper is to characterize eight extended-spectrum-ß-lactamase (ESBL) and acquired AmpC ß-lactamase-producing E. coli isolates and virotypes associated, obtained from chicken and pork food samples in Puebla, Mexico.
Design/methodology/approach
Samples (36 from chicken and 10 from pork) were cultured on Levine agar plates supplemented with cefotaxime (2 mg/L) for isolation of cefotaxime-resistant (CTXR) E. coli. CTXR-E. coli isolates were detected in 33 of 46 samples (72 percent), and one isolate/sample was characterized (28 from chicken and 5 from pork), for ESBL production, phylogenetic group, sequence typing, resistance and virulence genes by PCR and sequencing.
Findings
Results showed 16 ESBL-E. coli (35 percent) (12/16 belonging to phylogroup B1) and 8 CMY-2-E. coli (17 percent). ESBL detected were as follows (number of isolates): CTX-M-2 (8); CTX-M-1 (2); CTX-M-15 (1); SHV-2a (4) and TEM-52c (1). In total, 20 different sequence types (STs) were identified among the ESBL- or CMY-2-producing E. coli strains, which included four new ones. The CTX-M-15 β-lactamase was detected in one E. coli ST617-ST10 Cplx-B1 strain that also carried ibeA gene. One CMY-2-positive strain of lineage ST224-B2 was detected and it carried the qnrA1 gene.
Originality/value
In this study, a ST131-based virotyping scheme for strains from food of animal origin was established since this kind of strains constitutes an important vehicle of virulent ESBL- and CMY-2-producing E. coli isolates, which could be transmitted to humans by direct contact or through the food chain.
Keywords
Acknowledgements
Conflict of interest: competing interests – none declared.
Ethical approval: not required
This work was supported by the VIEP of the Benemérita Universidad Autónoma de Puebla (ROGR/NAT17-G) and for CONACYT México (reference 178942). EBV and GCC had a scholarship from CONACYT (Nos 411957 and 233611, respectively).
Citation
Barrios-Villa, E., Cortés-Cortés, G., Lozano Zarain, P., Romero-Romero, S., Lara Flores, N., Estepa, V., Somalo, S., Torres, C. and Rocha-Gracia, R.d.C. (2018), "Characterization of extended-spectrum and CMY-2
Publisher
:Emerald Publishing Limited
Copyright © 2018, Emerald Publishing Limited