Modification of poly (styrene-co-divinyl benzene) porous materials for peptides enrichment and separation
ISSN: 0369-9420
Article publication date: 6 August 2018
Issue publication date: 25 October 2018
Abstract
Purpose
The purpose of this paper is to focus on the selection of the optimum porous material modified with poly-dopamine coating for peptides enrichment. The adsorption behaviors for peptides and the antioxidant capacity of peptides fraction purified by the porous materials were investigated.
Design/methodology/approach
The optimum porous material with the highest adsorption capacity for peptides was selected for surface modification. The surface modified porous material was characterized by SEM, nitrogen adsorption-desorption isotherm and color change.
Findings
The results showed that the porous material was successfully modified with poly-dopamine coating. Adsorption capacity for peptides of the modified porous material was enhanced compared to the original porous material. Antioxidant capacity of peptides fraction enriched by the modified porous material was much higher than that enriched by the original porous material, indicating that the introduction of poly-dopamine coating was inclined to enrich peptides with certain amino acid residues.
Research limitations/implications
The structures of peptides are a bit not clear, which is the subject of future investigation.
Practical implications
This contribution provides a method to design and prepare porous materials with poly-dopamine coating to separate and enrich peptides or peptides fraction with high antioxidant capacity.
Originality/value
It showed that polarity, surface area, pore diameter and interactions were contributed to high adsorption capacity. The peptides fraction purified by the modified porous material showed excellent antioxidant capacity through results of reduction of DPPH radical, because of the enrichment of the peptides with certain amino acids residues which were considered to enhance radical scavenging capacity. This paper provides new insights into designing and preparing porous materials with poly-dopamine coating to enrich peptides fraction with high antioxidant capacity.
Keywords
Acknowledgements
The authors acknowledge the financial support of this work by the West Light Foundation of the Chinese Academy of Sciences, Natural Science Foundation of Gansu Province of China (1501RJZA011) and National Natural Sciences Foundation of China (NSFC No. 21605150 and 21544013).
Citation
Liu, Y., Wang, H., Liu, Y., Shi, M. and Di, D. (2018), "Modification of poly (styrene-co-divinyl benzene) porous materials for peptides enrichment and separation", Pigment & Resin Technology, Vol. 47 No. 5, pp. 406-414. https://doi.org/10.1108/PRT-05-2017-0048
Publisher
:Emerald Publishing Limited
Copyright © 2018, Emerald Publishing Limited