3D bioprinting of tissue engineering scaffold for cell culture

Abstract

Purpose

The 3 D bioprinting technology is used to prepare the tissue engineering scaffold with precise structures for the cell proliferation and differentiation.

Design/methodology/approach

According to the characteristics of the ideal tissue engineering scaffold, the microstructural design of the tissue engineering scaffold is carried out. The bioprinter is used to fabricate the tissue engineering scaffold with different structures and spacing sizes. Finally, the scaffold with good connectivity is achieved and used to cell PC12 culture.

Findings

The results show that the pore structure with the line spacing of 1 mm was the best for cell culture, and the survival rate of the inoculated cells PC12 is as high as 90%. The influence of the pore shape on the cell survival is not evidence.

Originality/value

This study shows that tissue engineering scaffolds prepared by 3 D bioprinting have graded structure for three-dimensional cell culture, which lays the foundation for the later detection of drug resistance.

Citation

Wu, L., Li, X., Guan, T., Chen, Y. and Qi, C. (2020), "3D bioprinting of tissue engineering scaffold for cell culture", Rapid Prototyping Journal, Vol. 26 No. 5, pp. 835-840. https://doi.org/10.1108/RPJ-10-2018-0264