A review of gel permeation chromatography: Part 3

Abstract

(b) Linear Homopolymers Linear poly(vinyl acetate) and polybutadiene samples were studied by Park and Graessley using GPC combined with capillary viscometry which provided both the distribution of hydrodynamic volumes and intrinsic viscosities of the samples. Their equipment comprised a Waters Associates Chromatograph (model 200) fitted with five Styragel columns of porosities 106, 105, 1.5 × 104, 104 and 3 and 103 mm eluted with THF at room temperature at a flow rate of 1 cm3 min−1. Samples of 2 cm3 nominal volume were injected at a concentration of 0.25 g per 100 cm3 and detection was by a differential refractometer coupled to a digital curve translator so that readings were digitised and fed to a teletype machine at one minute intervals. From the refractometer, the sample passed via a syphon device into an all glass viscometer of the Ubbelohde type and the whole of this equipment was totally immersed in a water bath whose volume was sufficient to smooth out minor temperature variations in the equipment. The viscometer discharge was detected photoelectrically and the time taken determined by an electronic timer which gave a signal that was also recorded on the teletype.

Citation

Ellis, R.A. (1979), "A review of gel permeation chromatography: Part 3", Pigment & Resin Technology, Vol. 8 No. 11, pp. 17-21. https://doi.org/10.1108/eb041529